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KMID : 0828520080120040235
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Journal of the Korean Geriatrics Society
2008 Volume.12 No. 4 p.235 ~ p.245
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Expression Change of Complement Regulator Genes of the Human Astrocytoma Cell Line after Aβ1-42 and Interferon Gamma Administration
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Choi Young-Sook
Kim Sang-Ho
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Abstract
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Background: We determined the changes of complement regulator gene expression in the amyloid-β1-42 (Aβ1-42) and interferon-gamma (IFN-γ)-stimulated human astrocytoma cell line.
Methods: The human astrocytoma cell line, U373MG , was stimulated with IFN-γ (62.5-1,000 U/m l) in the presence or absence of aggregated Aβ1-42 (1-20 μM ) for 24 hours. Messenger RNA expression of C1 inhibitor (C1-INH), complement factor I (CFI), clusterin, vitronectin, decay accelerating factor (DAF), membrane cofactor protein (MCP), and CD59 was measured by quantitative real-time reverse transcriptase-PCR .
Results: IFN-γ (final concentration, 500U/ml) m arkedly increased the expression of mRNA for C1-INH in a timedependent fashion. Aβ1-42 (final concentration, 2 μM ) induced a slight increase in the expression of C1-INH. Messenger RNAs for CFI and clusterin were m inim ally increased, but other regulators w ere unchanged or decreased by either Aβ1-42 or IFN-γ. IFN-γoverrode Aβ1-42-induced mRNA expression of C1-INH when the cells were treated with these two reagents together.
Conclusion: Among the complement regulator genes in the human astrocytoma cell line, U373MG , only C1-INH was significantly up-regulated by IFN-γ with or without Aβ1-42 administration.
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KEYWORD
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Alzheimers Disease, beta-amyloid protein, C1 Inhibitor, Complement Factor I, Interferon-gamma
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